rps7 sc 377317 Search Results


rps7  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology rps7
Rps7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cpsf1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology cpsf1
Proximity-dependent labeling and pull-down identify the association between mutant nsp1 and cellular proteins. (A) BioID2-fused nsp1 proteins carrying mutations in the middle region of nsp1 were expressed, treated with 100 μM biotin for 16 hours and biotinylated proteins were isolated with streptavidin-coated magnetic bead. These proteins were separated in SDS gel followed by the analysis of specific protein association using immunoblot. (B) The above experiment was repeated with nsp1 carrying mutations in the structurally flexible C-terminus of the protein followed by immunoblot. C-terminus mutations weaken association by <t>CPSF1,</t> NUDT21, eIF4G.
Cpsf1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7+sc+377317/CPSF1+Antibody/pmc08580555-198-7-4
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anti odc1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti odc1
Proximity-dependent labeling and pull-down identify the association between mutant nsp1 and cellular proteins. (A) BioID2-fused nsp1 proteins carrying mutations in the middle region of nsp1 were expressed, treated with 100 μM biotin for 16 hours and biotinylated proteins were isolated with streptavidin-coated magnetic bead. These proteins were separated in SDS gel followed by the analysis of specific protein association using immunoblot. (B) The above experiment was repeated with nsp1 carrying mutations in the structurally flexible C-terminus of the protein followed by immunoblot. C-terminus mutations weaken association by <t>CPSF1,</t> NUDT21, eIF4G.
Anti Odc1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7+sc+377317/ODC+Antibody/pmc06887119-257-69-71
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anti ccnd3  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti ccnd3
Proximity-dependent labeling and pull-down identify the association between mutant nsp1 and cellular proteins. (A) BioID2-fused nsp1 proteins carrying mutations in the middle region of nsp1 were expressed, treated with 100 μM biotin for 16 hours and biotinylated proteins were isolated with streptavidin-coated magnetic bead. These proteins were separated in SDS gel followed by the analysis of specific protein association using immunoblot. (B) The above experiment was repeated with nsp1 carrying mutations in the structurally flexible C-terminus of the protein followed by immunoblot. C-terminus mutations weaken association by <t>CPSF1,</t> NUDT21, eIF4G.
Anti Ccnd3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti hras  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti hras
Proximity-dependent labeling and pull-down identify the association between mutant nsp1 and cellular proteins. (A) BioID2-fused nsp1 proteins carrying mutations in the middle region of nsp1 were expressed, treated with 100 μM biotin for 16 hours and biotinylated proteins were isolated with streptavidin-coated magnetic bead. These proteins were separated in SDS gel followed by the analysis of specific protein association using immunoblot. (B) The above experiment was repeated with nsp1 carrying mutations in the structurally flexible C-terminus of the protein followed by immunoblot. C-terminus mutations weaken association by <t>CPSF1,</t> NUDT21, eIF4G.
Anti Hras, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7+sc+377317/H-Ras+Antibody/pmc06887119-257-59-61
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anti rps6  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti rps6
Proximity-dependent labeling and pull-down identify the association between mutant nsp1 and cellular proteins. (A) BioID2-fused nsp1 proteins carrying mutations in the middle region of nsp1 were expressed, treated with 100 μM biotin for 16 hours and biotinylated proteins were isolated with streptavidin-coated magnetic bead. These proteins were separated in SDS gel followed by the analysis of specific protein association using immunoblot. (B) The above experiment was repeated with nsp1 carrying mutations in the structurally flexible C-terminus of the protein followed by immunoblot. C-terminus mutations weaken association by <t>CPSF1,</t> NUDT21, eIF4G.
Anti Rps6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7+sc+377317/Ribosomal+Protein+S6+Antibody/pmc06887119-257-25-27
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anti eif4b  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology anti eif4b
Proximity-dependent labeling and pull-down identify the association between mutant nsp1 and cellular proteins. (A) BioID2-fused nsp1 proteins carrying mutations in the middle region of nsp1 were expressed, treated with 100 μM biotin for 16 hours and biotinylated proteins were isolated with streptavidin-coated magnetic bead. These proteins were separated in SDS gel followed by the analysis of specific protein association using immunoblot. (B) The above experiment was repeated with nsp1 carrying mutations in the structurally flexible C-terminus of the protein followed by immunoblot. C-terminus mutations weaken association by <t>CPSF1,</t> NUDT21, eIF4G.
Anti Eif4b, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti eif3g  (St Johns Laboratory)
90
St Johns Laboratory anti eif3g
( a ) Patterns of protein crosslinking generated with the indicated mRNAs, which are labeled with 4-thio-U and [α- 32 P]-GTP. The identified protein bands are indicated, with a relative quantification of the intensity of the protein bands shown below, according to this experiment and the one shown in . ( b ) Mapping of the interaction of eIF4A and <t>eIF3g</t> with mRNA in the ES6S region. SFV-DLP mRNAs with a single 4-thio-U placed at the indicated positions were used to assemble the 48S-PIC, and the pattern of protein crosslinking was analyzed. ( c ) The positions of mRNA that crosslinked with eIF4A and eIF3g were projected on the solvent side of the PIC, assuming that the mRNA is threaded along the ES6S region. The +11 position of mRNA is placed at the mRNA entry channel ( ; ). A rise per base of 4.5 Å for a stretched RNA strand was used as described previously .
Anti Eif3g, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7+sc+377317/Anti-EIF3G+Antibody/pmc06887119-257-35-37
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anti akt1  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc anti akt1
( a ) Patterns of protein crosslinking generated with the indicated mRNAs, which are labeled with 4-thio-U and [α- 32 P]-GTP. The identified protein bands are indicated, with a relative quantification of the intensity of the protein bands shown below, according to this experiment and the one shown in . ( b ) Mapping of the interaction of eIF4A and <t>eIF3g</t> with mRNA in the ES6S region. SFV-DLP mRNAs with a single 4-thio-U placed at the indicated positions were used to assemble the 48S-PIC, and the pattern of protein crosslinking was analyzed. ( c ) The positions of mRNA that crosslinked with eIF4A and eIF3g were projected on the solvent side of the PIC, assuming that the mRNA is threaded along the ES6S region. The +11 position of mRNA is placed at the mRNA entry channel ( ; ). A rise per base of 4.5 Å for a stretched RNA strand was used as described previously .
Anti Akt1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7+sc+377317/Akt+Antibody/pmc06887119-257-47-49
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anti eef1a1  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc anti eef1a1
( a ) Patterns of protein crosslinking generated with the indicated mRNAs, which are labeled with 4-thio-U and [α- 32 P]-GTP. The identified protein bands are indicated, with a relative quantification of the intensity of the protein bands shown below, according to this experiment and the one shown in . ( b ) Mapping of the interaction of eIF4A and <t>eIF3g</t> with mRNA in the ES6S region. SFV-DLP mRNAs with a single 4-thio-U placed at the indicated positions were used to assemble the 48S-PIC, and the pattern of protein crosslinking was analyzed. ( c ) The positions of mRNA that crosslinked with eIF4A and eIF3g were projected on the solvent side of the PIC, assuming that the mRNA is threaded along the ES6S region. The +11 position of mRNA is placed at the mRNA entry channel ( ; ). A rise per base of 4.5 Å for a stretched RNA strand was used as described previously .
Anti Eef1a1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7+sc+377317/eEF1A+Antibody/pmc06887119-257-43-45
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mouse monoclonal antibody  (Proteintech)
96
Proteintech mouse monoclonal antibody
( a ) Patterns of protein crosslinking generated with the indicated mRNAs, which are labeled with 4-thio-U and [α- 32 P]-GTP. The identified protein bands are indicated, with a relative quantification of the intensity of the protein bands shown below, according to this experiment and the one shown in . ( b ) Mapping of the interaction of eIF4A and <t>eIF3g</t> with mRNA in the ES6S region. SFV-DLP mRNAs with a single 4-thio-U placed at the indicated positions were used to assemble the 48S-PIC, and the pattern of protein crosslinking was analyzed. ( c ) The positions of mRNA that crosslinked with eIF4A and eIF3g were projected on the solvent side of the PIC, assuming that the mRNA is threaded along the ES6S region. The +11 position of mRNA is placed at the mRNA entry channel ( ; ). A rise per base of 4.5 Å for a stretched RNA strand was used as described previously .
Mouse Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7+sc+377317/VEGFA+Monoclonal+antibody/ppr0772292-523-0-6
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α 665 tubulin  (Proteintech)
96
Proteintech α 665 tubulin
( a ) Patterns of protein crosslinking generated with the indicated mRNAs, which are labeled with 4-thio-U and [α- 32 P]-GTP. The identified protein bands are indicated, with a relative quantification of the intensity of the protein bands shown below, according to this experiment and the one shown in . ( b ) Mapping of the interaction of eIF4A and <t>eIF3g</t> with mRNA in the ES6S region. SFV-DLP mRNAs with a single 4-thio-U placed at the indicated positions were used to assemble the 48S-PIC, and the pattern of protein crosslinking was analyzed. ( c ) The positions of mRNA that crosslinked with eIF4A and eIF3g were projected on the solvent side of the PIC, assuming that the mRNA is threaded along the ES6S region. The +11 position of mRNA is placed at the mRNA entry channel ( ; ). A rise per base of 4.5 Å for a stretched RNA strand was used as described previously .
α 665 Tubulin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rps7+sc+377317/alpha+Tubulin+Antibody/ppr0772292-523-4-6
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Image Search Results


Proximity-dependent labeling and pull-down identify the association between mutant nsp1 and cellular proteins. (A) BioID2-fused nsp1 proteins carrying mutations in the middle region of nsp1 were expressed, treated with 100 μM biotin for 16 hours and biotinylated proteins were isolated with streptavidin-coated magnetic bead. These proteins were separated in SDS gel followed by the analysis of specific protein association using immunoblot. (B) The above experiment was repeated with nsp1 carrying mutations in the structurally flexible C-terminus of the protein followed by immunoblot. C-terminus mutations weaken association by CPSF1, NUDT21, eIF4G.

Journal: The Journal of Biological Chemistry

Article Title: Proximity-dependent biotinylation detects associations between SARS coronavirus nonstructural protein 1 and stress granule-associated proteins

doi: 10.1016/j.jbc.2021.101399

Figure Lengend Snippet: Proximity-dependent labeling and pull-down identify the association between mutant nsp1 and cellular proteins. (A) BioID2-fused nsp1 proteins carrying mutations in the middle region of nsp1 were expressed, treated with 100 μM biotin for 16 hours and biotinylated proteins were isolated with streptavidin-coated magnetic bead. These proteins were separated in SDS gel followed by the analysis of specific protein association using immunoblot. (B) The above experiment was repeated with nsp1 carrying mutations in the structurally flexible C-terminus of the protein followed by immunoblot. C-terminus mutations weaken association by CPSF1, NUDT21, eIF4G.

Article Snippet: Antibodies were purchased from Santa Cruz Biotechnology: CPSF1 (G10, sc-166281), CPSF7 (A9, sc-393880), Xrn2 (H3, sc-365258), Hsp70 (B6, sc-7298), G3BP (H10, sc-363338), Rps7 (E1, sc-377317), NUDT21 (F5, sc-515766), eIF4G (A10, sc-133155), GAPDH (FL-335, sc-25778), and Sigma-Aldrich: phosphorylated eIF2α (SAB5700436), PABPC1 (HPA045423); Abcam ATXN2L (ab-99304), TIA1-40kDa (ab-263945); Thermo Fisher: USP10 (PA5-52334).

Techniques: Labeling, Mutagenesis, Isolation, SDS-Gel, Western Blot

( a ) Patterns of protein crosslinking generated with the indicated mRNAs, which are labeled with 4-thio-U and [α- 32 P]-GTP. The identified protein bands are indicated, with a relative quantification of the intensity of the protein bands shown below, according to this experiment and the one shown in . ( b ) Mapping of the interaction of eIF4A and eIF3g with mRNA in the ES6S region. SFV-DLP mRNAs with a single 4-thio-U placed at the indicated positions were used to assemble the 48S-PIC, and the pattern of protein crosslinking was analyzed. ( c ) The positions of mRNA that crosslinked with eIF4A and eIF3g were projected on the solvent side of the PIC, assuming that the mRNA is threaded along the ES6S region. The +11 position of mRNA is placed at the mRNA entry channel ( ; ). A rise per base of 4.5 Å for a stretched RNA strand was used as described previously .

Journal: eLife

Article Title: An mRNA-binding channel in the ES6S region of the translation 48S-PIC promotes RNA unwinding and scanning

doi: 10.7554/eLife.48246

Figure Lengend Snippet: ( a ) Patterns of protein crosslinking generated with the indicated mRNAs, which are labeled with 4-thio-U and [α- 32 P]-GTP. The identified protein bands are indicated, with a relative quantification of the intensity of the protein bands shown below, according to this experiment and the one shown in . ( b ) Mapping of the interaction of eIF4A and eIF3g with mRNA in the ES6S region. SFV-DLP mRNAs with a single 4-thio-U placed at the indicated positions were used to assemble the 48S-PIC, and the pattern of protein crosslinking was analyzed. ( c ) The positions of mRNA that crosslinked with eIF4A and eIF3g were projected on the solvent side of the PIC, assuming that the mRNA is threaded along the ES6S region. The +11 position of mRNA is placed at the mRNA entry channel ( ; ). A rise per base of 4.5 Å for a stretched RNA strand was used as described previously .

Article Snippet: Western blots were carried out as described previously ( ) using the following primary antibodies: anti-RPS4X (sc-85133, Santa Cruz Biotech.), anti-RPS7 (sc-377317, Santa Cruz Biotech.), anti-RPS6 (sc-74576, Santa Cruz Biotech.), anti-eIF4A (STJ2724, St. John´s lab,), anti-eIF3g (STJ23512, St. John´s lab), anti-EGFP (11814460001, Roche), anti-eEF1A1 (2551, Cell Signaling), anti-AKT1 (9272, Cell Signaling), anti-ACTB (T-5168, Sigma), anti-eIF4b (sc-376062, Santa Cruz Biotech.), anti-HRas (sc-53959, Santa Cruz Biotech.), anti-CCND3 (sc-453, Santa Cruz Biotech.), anti-ODC1 (sc-398116, Santa Cruz Biotech.), anti-GRK2 (a gift from C. Murga, CBMSO).

Techniques: Generated, Labeling

( a ) Denaturing immunoprecipitation (dIP) of crosslinked 48S-PIC revealed the presence of eIF4A and eIF3g bound to the indicated mRNAs (arrowheads). ( b ) Western blot analysis showing the specificity of the eIF4A1 antibody. HeLa cells were transfected with the indicated esiRNA, and 72 hr later, the resulting cells extracts were probed with anti-eIF4A1 and anti-eIF2 antibodies. esiRNA against the human ZC3HAV1 gene was used as control.

Journal: eLife

Article Title: An mRNA-binding channel in the ES6S region of the translation 48S-PIC promotes RNA unwinding and scanning

doi: 10.7554/eLife.48246

Figure Lengend Snippet: ( a ) Denaturing immunoprecipitation (dIP) of crosslinked 48S-PIC revealed the presence of eIF4A and eIF3g bound to the indicated mRNAs (arrowheads). ( b ) Western blot analysis showing the specificity of the eIF4A1 antibody. HeLa cells were transfected with the indicated esiRNA, and 72 hr later, the resulting cells extracts were probed with anti-eIF4A1 and anti-eIF2 antibodies. esiRNA against the human ZC3HAV1 gene was used as control.

Article Snippet: Western blots were carried out as described previously ( ) using the following primary antibodies: anti-RPS4X (sc-85133, Santa Cruz Biotech.), anti-RPS7 (sc-377317, Santa Cruz Biotech.), anti-RPS6 (sc-74576, Santa Cruz Biotech.), anti-eIF4A (STJ2724, St. John´s lab,), anti-eIF3g (STJ23512, St. John´s lab), anti-EGFP (11814460001, Roche), anti-eEF1A1 (2551, Cell Signaling), anti-AKT1 (9272, Cell Signaling), anti-ACTB (T-5168, Sigma), anti-eIF4b (sc-376062, Santa Cruz Biotech.), anti-HRas (sc-53959, Santa Cruz Biotech.), anti-CCND3 (sc-453, Santa Cruz Biotech.), anti-ODC1 (sc-398116, Santa Cruz Biotech.), anti-GRK2 (a gift from C. Murga, CBMSO).

Techniques: Immunoprecipitation, Western Blot, Transfection, esiRNA

The amount of [ 32 P]-mRNA bound to WRF is indicated (upper panel), showing the pattern of protein crosslinking with the indicated mRNAs in the absence and presence of VIC–oligo 4. The unresolved eIF3g and eIF4A bands are shown (lower panel).

Journal: eLife

Article Title: An mRNA-binding channel in the ES6S region of the translation 48S-PIC promotes RNA unwinding and scanning

doi: 10.7554/eLife.48246

Figure Lengend Snippet: The amount of [ 32 P]-mRNA bound to WRF is indicated (upper panel), showing the pattern of protein crosslinking with the indicated mRNAs in the absence and presence of VIC–oligo 4. The unresolved eIF3g and eIF4A bands are shown (lower panel).

Article Snippet: Western blots were carried out as described previously ( ) using the following primary antibodies: anti-RPS4X (sc-85133, Santa Cruz Biotech.), anti-RPS7 (sc-377317, Santa Cruz Biotech.), anti-RPS6 (sc-74576, Santa Cruz Biotech.), anti-eIF4A (STJ2724, St. John´s lab,), anti-eIF3g (STJ23512, St. John´s lab), anti-EGFP (11814460001, Roche), anti-eEF1A1 (2551, Cell Signaling), anti-AKT1 (9272, Cell Signaling), anti-ACTB (T-5168, Sigma), anti-eIF4b (sc-376062, Santa Cruz Biotech.), anti-HRas (sc-53959, Santa Cruz Biotech.), anti-CCND3 (sc-453, Santa Cruz Biotech.), anti-ODC1 (sc-398116, Santa Cruz Biotech.), anti-GRK2 (a gift from C. Murga, CBMSO).

Techniques: